Preconditioning of Human Dental Pulp Stem Cells with Leukocyte- and Platelet-Rich Fibrin-Derived Factors Does Not Enhance Their Neuroregenerative Effect

Figure 3

Metabolic and proliferative activity of hDPSCs exposed to CM and EX L-PRF and the effect of L-PRF priming on hDPSC-derived BDNF secretion. The metabolic (a, c) and proliferative (b, d) effect of hDPSCs that were exposed for 24 h, 48 h, and 72 h to CM (a, b) or EX L-PRF (c, d) was evaluated by means of an MTT and PI assay, respectively (). CM and EX L-PRF had an inverse effect on hDPSC metabolism as high concentrations of CM significantly diminished, and high concentrations of EX L-PRF significantly stimulated hDPSC metabolism. Both CM and EX L-PRF had a dose-response effect on proliferation as higher concentrations significantly enhanced hDPSC proliferation. BDNF secretion (e) was increased in hDPSCs primed () with 10% CM L-PRF or 5% and 10% EX L-PRF for 24 h while significant BDNF secretion could only be observed when hDPSCs were primed for 48 h with 10% CM or EX L-PRF. value ≤ 0.05, value ≤ 0.01, and value ≤ 0.001. Data are expressed as .

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