CD73 Expression on Mesenchymal Stem Cells Dictates the Reparative Properties via Its Anti-Inflammatory Activity

Figure 4

The obligatory role of CD73 and adenosine in the regulation of macrophage activity. When cocultivated with CD73^high pMSC, rat peritoneal macrophages (pMϕ) strikingly upregulated several anti-inflammatory cytokines, including IL-10, Arg-1, and VEGF, and downregulated proinflammatory cytokines, including TNF-α and IFN-γ, in comparison to the placebo control, while pMϕ cocultivated with CD73^-/- pMSC did not significantly alter the cytokine profile (a). Pretreatment of pMϕ with the selective antagonist of the A2b receptor of adenosine, PSB603, partially blocked the upregulation of IL-10 induced by CD73 pMSC (b) and restored the inhibitory effects on the TNF-α expression (c). indicates and indicates . # indicates compared to CD73^high pMSC.

(a)

(b)

(c)