Protection of the Peritoneal Membrane by Peritoneal Dialysis Effluent-Derived Mesenchymal Stromal Cells in a Rat Model of Chronic Peritoneal Dialysis

Figure 4

Incubation with pMSC-conditioned medium (pMSC-CM) inhibits H₂O₂-induced apoptosis in cultured human peritoneal mesothelial cells (HPMCs). HPMCs ( cells/well) were grown in 24-well plates overnight, followed by treatment with 0.1 μM H₂O₂ in the absence or presence of 25% or 50% (/) of pMSC-CM for 24 h. Cell apoptosis was determined by flow cytometric analysis with Annexin-V-PE and 7-AAD staining. (a) A typical dot plot showing the percentage of Annexin-V-PE- and/or 7-AAD-positive staining of cell populations in each group. (b) Apoptosis was measured by the sum of Annexin V-stained cell populations (Annexin-V-PE positive and Annexin-V-PE and 7-AAD double positive in the right lower and upper quadrants). Data are presented as of seven determinants and were statistically analyzed by using the two-tailed -test. (H₂O₂ vs. medium) and (pMSCs+25% or 50% vs. H₂O₂).

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