Research Article

Wnt-GSK3β/β-Catenin Regulates the Differentiation of Dental Pulp Stem Cells into Bladder Smooth Muscle Cells

Figure 3

The involvement of Wnt-mediated GSK3β/β-catenin in SMC differentiation from DPSC clone A32. The A32 clone was induced according to the SMC induction protocol, 20% conditioned medium, and 2.5 ng/mL TGF-β1 for the indicated time (0 d, 5 d, 8 d, 11 d, and 14 d). The protein levels of p-GSK3β, t-GSK3β, and β-catenin were analysed with western blotting (a and c). The relative band intensities were determined by densitometry (b and d). Statistical analysis was performed by using one-way ANOVA. Date are shown as . when compared with the 0 d group.
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