Does the Harvesting Site Influence the Osteogenic Potential of Mesenchymal Stem Cells?

Figure 1

Cell morphology, proliferation, and marker expression in monolayer culture. (a) Morphology of the cells in culture at day 21. The middle and the right photos show the cells released from the bone chips (on the top left and bottom left corner, respectively) and expanding, scale bar 50 μm. (b) Cell colonies stained with crystal violet solution after plating 14 days showing a 1.5- and 1.6-fold increase in the cells derived from the acetabulum compared with the cells from the bone marrow and femur, respectively. Data are shown by the of at least four independent experiments performed in triplicate from at least four different primary cultures, . (c) Proliferation curve of the cells in adherent culture determined by measuring absorbance of the cells stained with crystal violet at 570 nm wavelength. Results are the of at least three experiments done in quintuplicate from at least three different cell cultures (, ). (d) Morphology of cells stained with crystal violet in culture at day 6, scale bar 50 μm. (e) Charts showing cell marker expression analyzed by flow cytometry. The expression percentage of the antigens is the from three independent experiments of three different primary cell cultures ().

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