Does the Harvesting Site Influence the Osteogenic Potential of Mesenchymal Stem Cells?

Figure 2

Adipogenic differentiation. Confluent cells were induced in adipogenic medium (AM) for three weeks to differentiate into adipocytes; the control (CTR) cells were maintained in complete medium. (a) Oil Red O stain. Cells were stained with Oil Red O solution, which marks fat droplets into red color. Photos of the CTR cells are not shown, scale bar 50 μm. Quantification was determined by measuring the absorbance of the stained cells at 510 nm (, , , ). (b) qPCR results. Expression of the adipocytic marker genes LPL and PPARG of the cells cultured in CTR and AM conditions. Data are presented as value. The lowest charts show the expression comparison of these markers among the cells originated from all three sources (, , ).

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(b)