Isolation, Characterization, and Safety Evaluation of Human Skin-Derived Precursors from an Adherent Monolayer Culture System

Figure 13

Analysis of biological safety for FBs and tSKPs. (a) RT-PCR analysis for the expression of HLA-I, HLA-DR, and, as a control, GADPH in FBs and tSKPs that had been passaged five and ten times. (b) Flow cytometer analysis for the surface marker expression of HLA-I, HLA-DR, and, as a control, GADPH in FBs and tSKPs that had been passaged five and ten times. (c) Effect of FBs and tSKPs to SCID mice on anatomic pathology. HE staining revealed that all organs from different groups showed normal histology. (d) Effect of FBs and tSKPs to SCID mice on the subpopulation of T lymphocytes. No significant change in the subpopulation of T lymphocytes. The values are expressed as .

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