Isolation, Characterization, and Safety Evaluation of Human Skin-Derived Precursors from an Adherent Monolayer Culture System

Figure 4

Multiple differentiation potentials of tSKPs generated from primary adherent FBs. (a) tSKPs could differentiate into adipocytes after induction for 28 days. (A) Phase contrast imaging showed lipid droplet inclusions, (B) and these lipids were positive staining for Oil Red-O. (C) The qRT-PCR results showed that PPAR-γ and FABP-4 was significantly increased after induction. (b) tSKPs could differentiate into osteocytes after induction for 28 days. (A) Calcium deposition was detected by Alizarin Red staining. (B) The qRT-PCR results showed that Runx2 was significantly increased after induction. (c) tSKPs could differentiate into smooth muscle cells after induction for 28 days. (A) Phase contrast imaging revealed the morphology of elongated and spindle appearance. The immunocytochemistry analysis showed that cells were positive for (B) α-SMA and (C) Calponin. (D) The qRT-PCR results showed that α-SMA was significantly increased after induction. (d) tSKPs could differentiate into Schwann cells after induction for 28 days. (A) The immunofluorescence staining revealed the expression of the Schwann cell differentiation marker of S100β. (B) The qRT-PCR results showed that S100β and GFAP were significantly increased after induction. (e) After induction in a neuron differentiation medium for 28 days, (A) immunofluorescence staining detected that cells were negative for βIII-tubulin, while the mRNA expression of βIII-tubulin was significantly increased after induction. , . Scale bars: 100 μm.

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