Isolation, Characterization, and Safety Evaluation of Human Skin-Derived Precursors from an Adherent Monolayer Culture System

Figure 5

tSKP spheres generated from FBs contain aggregated cells. (a) tSKPs generated from mixed FBs expressing either EGFP or mCherry equally with a seeding density of cells/mL. Phase contrast (A) and fluorescence photomicrograph (D) of tSKPs five days after transculture. Immunofluorescence detection of EGFP- (green) (B) and mCherry-positive cells (red) (C) in tSKP spheres, respectively. Arrows indicated spheres containing both EGFP- and mCherry-positive cells, and arrowheads showed spheres consisted of cells expressing either EGFP or mCherry alone. (b) Formation of tSKP spheres derived from mixed-FB plating at different densities of , , , , , and cells/mL. (c) Enlarge the red box of (b). Arrows showed the spheres formed at a density of and cells/mL consisted of both EGFP- and mCherry-positive cells in day 7. Scale bars: 100 μm.

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