Pancreatic Progenitors and Organoids as a Prerequisite to Model Pancreatic Diseases and Cancer

Figure 1

Stages of in vitro pancreatic development generating endocrine cells. (a) Stepwise differentiation of the human ESC line HUES8 with representative phase contrast images of the respective pancreatic lineage with 2D culture from the PSC stage until the PP stage, sphere culture at β-cell stage. (b) Immunofluorescence staining of cells from β-cell stage for C-peptide (β-cell marker, green) and glucagon (α-cell marker, red).

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