Pancreatic Progenitors and Organoids as a Prerequisite to Model Pancreatic Diseases and Cancer

Figure 3

Schematic overview of stepwise pancreatic endocrine differentiation protocols. Stage-specific modulation is conducted by the addition of different growth factors and small molecules. The duration of each stage is depicted in days. PSC: pluripotent stem cells; DE: definitive endoderm; PGT: primitive gut tube; PFG: posterior foregut; PE: pancreatic endoderm; EP: pancreatic endocrine precursors; i-β: immature β-cells; m-β: mature β-cells; PP1: PDX1⁺ pancreatic progenitors; PP2: PDX1⁺/NKX6.1⁺ pancreatic progenitors; EN: NKX6.1⁺/C-peptide⁺ cells.