Head to Knee: Cranial Neural Crest-Derived Cells as Promising Candidates for Human Cartilage Repair

Figure 3

Chondrogenic differentiation potential of human and mouse NC-MSC: gingival stem cell example. (a) Mouse gingival stem cells (GSCs) were cultured for 21 days in suspension under suitable chondrogenic conditions. (b) Human GSCs were cultured for 21 days in suspension under suitable chondrogenic conditions. Immunostaining of actin and nuclei (Dapi) confirmed the initial fibroblastic phenotype. (c) Immunostaining of Col2 and cell nuclei (Dapi) in mouse GSC confirmed ECM cartilage synthesis in the chondrogenic pellet. (d) Immunostaining of hyaluronan-binding protein (HABP) in the GSC pellet after 35 days of chondrogenic differentiation. (e, f) Immunostaining of Col10 in the GSC pellet after 21 days of chondrogenic differentiation, showing the inner layers with low and high magnifications.

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