Neuronal and glial differentiation of HF NCSCs and SD NCSCs. mRNA expression levels of neuronal (a) and glial (b) markers; qPCR analysis. The expression of all target genes was normalised to TBP gene expression levels and then to the undifferentiated control cells. The HF NCSC and SD NCSC cultures from five donors were analysed. (c–j) Immunocytochemical analysis of neuronal and glial (Schwann cells). (k, l) Marker expression by HF NCSCs (c, e, g, i, k) and SD NCSCs (d, f, h, j, l). (c, d) NeuN (green); (e, f) neurofilament (NF; green); (g, h) neuron-specific enolase (NSE; red); (i, j) βIII-tubulin (green); (k, l) S100β (green). The nuclei were stained with Hoechst 33342 dye; confocal microscopy. (c, d) Scale bar: 10 μm. (e–l) Scale bar: 20 μm.