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Figure 3: Three-dimensional growth of HF NCSC (a, b) and SD NCSC (c, d) in fibrin hydrogel. Fluorescein diacetate (FDA)/propidium iodide (PI) combined staining for live cells 24 h (a, c) and 72 h (b, d) after plating. (e) Diagram of the population doubling time (PDT) values for HF NCSCs and SD NCSCs populations grown in 2D vs. 3D cultures; initial plating: 104 cells per 35 mm Petri dish; culture period: 168 h; for cultures from five donors. between 2D and 3D HF; between 2D and 3D SD.