Research Article
Laminin as a Potent Substrate for Large-Scale Expansion of Human Induced Pluripotent Stem Cells in a Closed Cell Expansion System
Table 1
List of primers for pluripotency and germ layer differentiation.
| | Primer | Forward | Reverse |
| Pluripotency | OCT4 | CCATGCATTCAAACTGAGGTG | CCTTTGTGTTCCCAATTCCTTC | NANOG | CTCCAGGATTTTAACGTTCTGC | TGGGATAAAGTGAGTTGCCTG | LIN28 | AAGAAATCCACAGCCCTACC | CCCCCCTAACCCATCACCTCCACCACCTAA | SOX2 | GAGAAGTTTGAGCCCCAGG | AGAGGCAAACTGGAATCAGG | REX1 | CAGATCCTAAACAGCTCGCAGAAT | GCGTACGCAAATTAAAGTCCAGA | DPPA4 | CAGCTCTGCTCATGACTGTTG | ATAGTAGCTAGCTTTGATGGCA | NODAL | GGGCAAGAGGCACCGTCGACATCA | GGGACTCGGTGGGGCTGGTAACGTTTC | TDGFb | CTGCTGCCTGAATGGGGGAACCTGC | GCCACGAGGTGCTCATCCATCACAAGG | TERT3 | CCTGCTCAAGCTGACTCGACACCGTG | GGAAAAGCTGGCCCTGGGGTGGAGC | GDF3 | GTGCCAACCCAGGTCCGGAAGTT | CTTATGCTACGTAAAGGAGCTGGG |
| Ectoderm | NESTIN | CACCTCAAGATGTCCCTCAG | AGCAAAGATCCAAGACGCC | TUBB3 | GCTCAGGGGCCTTTGGACATCTCTT | TTTTCACACTCCTTCCGCACCACATC | NEFH | ACCTATACCCGAATGCCTTCTT | AGAAGCACTTGGTTTTATTGCAC |
| Mesoderm | MSX1 | CGAGAGGACCCCGTGGATGCAGAG | GGCGGCCATCTTCAGCTTCTCCAG | BMP4 | GCACTGGTCTTGAGTATCCTG | TGCTGAGGTTAAAGAGGAAACG | Brachyury (T) | GCCCTCTCCCTCCCCTCCACGCACAG | CGGCGCCGTTGCTCACAGACCACAGG |
| Endoderm | GATA6 | CCAACTGTCACACCACAAC | TGGGGGAAGTATTTTTGCTG | AFP | GAATGCTGCAAACTGACCACGCTGGAAC | TGGCATTCAAGAGGGTTTTCAGTCTGGA | SOX17 | GACGACCAGAGCCAGACC | CGCCTCGCCCTTCACC | GAPDH | AATCCCATCACCATCTTCCAG | AAATGAGCCCCAGCCTTC |
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