Noninvasive Monitoring of Allogeneic Stem Cell Delivery with Dual-Modality Imaging-Visible Microcapsules in a Rabbit Model of Peripheral Arterial Disease

Figure 5

Ex vivo validation of PFOBCap injection sites. (a) Gross anatomical view of the rabbit medial thigh shows engrafted PFOBCaps (arrows) three days after implantation without hemorrhage or gross fibrosis. (b) Segmented injection sites with each injection show as a different color from postmortem tissue. (c) A C-arm CT image of the same rabbit demonstrates the detection of all six injections of PFOBCaps in the right thigh. (d) Fusion of C-arm CT image (c) with volume rendering of postmortem injections from (b) demonstrates high correlation of PFOBCap locations in the rabbit thigh. (e) Recovered PFOBCap injection site from rabbit medial thigh demonstrates that PFOBCaps remained intact two weeks after delivery. (f) Individual, intact PFOBCap recovered from tissue chunk two weeks after delivery. Scale bars represent 200 μm.

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