Noninvasive Monitoring of Allogeneic Stem Cell Delivery with Dual-Modality Imaging-Visible Microcapsules in a Rabbit Model of Peripheral Arterial Disease

Figure 6

Histopathological analysis of MSC survival after transplantation. (a) Hematoxylin & eosin (H&E) staining of a rabbit receiving PFOBCaps with MSCsM shows absence of inflammatory cell infiltrate two weeks after delivery. (b) H&E staining of a rabbit receiving naked MSCs demonstrates no evidence of inflammatory infiltrates three days of postdelivery. (c) TUNEL staining reveals a few apoptotic MSCs (green, arrow) within PFOBCaps two weeks after transplantation. DAPI staining (blue) shows total cell nuclei. (d) qPCR analysis of Sry gene confirms the retention of male MSCs in rabbits up to three days of posttransplantation. Male rabbit MSCs serve as positive control (+) and female rabbit skeletal muscle cells serve as negative control (-). (e) TUNEL staining of CTO-labeled (orange) unencapsulated (or naked) MSCs reveals majority of cell death three days after delivery. Scale bars represent 200 μm in (a–c) and 100 μm in (e).

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