A Bioactive Cartilage Graft of IGF1-Transduced Adipose Mesenchymal Stem Cells Embedded in an Alginate/Bovine Cartilage Matrix Tridimensional Scaffold

Figure 1

Isolation and characterization of ADSCs from the adipose tissue of Ovis aries. (a) Monolayer of adherent spindle-shaped fibroblastoid cells compatible with undifferentiated mesenchymal stem cells. Culture of the adherent cell population at 24 h postisolation. Cells cultured with the DMEM after 7 days. The cell culture reached confluence after 21 days (scale bar = 80 μm). (b) Flow cytometry analyses are illustrated. FSC by SSC indicates a homogeneous population in size and granularity. (c) Surface marker expression: the data represent the mean deviation of 3 independent experiments. (d) Trilineage differentiation of ADSCs into adipocytes, osteoblast, and chondrocytes is shown. Oil red staining of ADSCs induced for adipocyte differentiation. Alcian blue staining of ABMCs induced for chondrocyte differentiation. Osteoblast differentiation visualized using Alizarin red (scale bar = 80 μm).

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