A Bioactive Cartilage Graft of IGF1-Transduced Adipose Mesenchymal Stem Cells Embedded in an Alginate/Bovine Cartilage Matrix Tridimensional Scaffold

Figure 5

SEM micrographs of nontransduced (2CI) and transduced (3CI) ADSCs grown on the alginate/bovine cartilage matrix scaffold. (a–c) Micrographs showing the ECM of decellularized bovine cartilage. (d–f) Micrographs showing the longitudinal views of the 2CI implant. (e) High magnification revealed cell adhesion and chondrocyte-like morphology. (f) Matrix synthesis and fibers around the cells after 28 days of culture. (g–i) Longitudinal views of the 3CI implant show the same characteristics as 2CI and also highlight the formation of typical isogenous groups (g and h) and filopodia-like structures (inset in h) after 28 days of culture.