A Bioactive Cartilage Graft of IGF1-Transduced Adipose Mesenchymal Stem Cells Embedded in an Alginate/Bovine Cartilage Matrix Tridimensional Scaffold

Table 1

Gene	Ref. no.	Sequence 5-3	Amplicon (bp)
Col-I	FJ200442.1	Fw: GGTGACAGGAAGTCCCAGAA	167
		Rv: CCATCGTAGGTGACGCTGTA
Col-II	X02420.1	Fw: CTACTGGATTGACCCCAACC	211
		Rv: TGTCCTTGCTCTTGCTGATG
Aggrecan (AGC)	NM_173981.2	Fw: CAGAGTTCAGTGGGACAGCA	189
		Rv: AGACACCCAGCTCTCCTGAA
Proteoglycan (PGC)	NM_174288.1	Fw: TGCTGTGATTGCCTCTTTTG	169
		Rv: CCAAAACCCGTAGTTCCTGA
Biglycan (BGC)	BT021201.1	Fw: ACCTCCCTGAGACCCTCAAT	184
		Rv: TTGTTGTCCAAGTGCAGCTC
Cartilage oligomeric matrix protein (COMP)	X74326.1	Fw: ATGCGGACAAGGTGGTAGAC	153
		Rv: TCTCCATACCCTGGTTGAGC
GAPDH	NM_001190390.1	Fw: CCATCACCATCTTCCAGGAGCG	481
		Rv: AAGGCCATGCCAGTGAGCTTC

The sequence was designed based on the Bos taurus genome since the Ovis aries genome was not assembled for this gene.