Microvesicles from Human Immortalized Cell Lines of Endothelial Progenitor Cells and Mesenchymal Stem/Stromal Cells of Adipose Tissue Origin as Carriers of Bioactive Factors Facilitating Angiogenesis

Figure 2

Characterization and internalization of microvesicles isolated from the EPC line (HEPC-CB.1 cell line (a)) and from adipose tissue HATMSC1 cell line (b). (a1 and b1) Isolated microvesicles were counted using fluorescent counting beads (left panel). Microvesicles were then assessed for the purity of isolation using dynamic light scattering (right panel). (a2 and b2) Representative images of microvesicle internalization into target cells: dermal microvascular endothelial cells HSkMEC.2, fibroblasts MSU 1.1, and keratinocytes HaCaT. Images were taken using an inverted microscope after 24 h of incubation with microvesicles dyed with the green fluorescent DiO dye (scale bar: 20 μm). (a3 and b3) Flow cytometric histograms showing green fluorescence of recipient cells after microvesicle internalization. Black histograms represent the control for untreated cells, and red histograms represent cells treated with labeled microvesicles.

(a)

(b)