Bone Defect Repair Using a Bone Substitute Supported by Mesenchymal Stem Cells Derived from the Umbilical Cord

Figure 6

WJ-MSC on the Bio-Oss® Collagen scaffold cultured in control medium and osteodifferentiation medium. Pictures from a confocal microscope (a)—cells were stained with calcein AM (alive cells—green staining) and ethidium homodimer (dead cells—red staining). Pictures from an electron microscope (b, c). Scale bar in (a) row: 100 μm. Image of the entire scaffold cultured with WJ-MSC in control (d) and osteodifferentiation (e) medium (green—calcein AM; red—ethidium homodimer). Scale bar: 1000 μm