Soluble PTX3 of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Attenuates Hyperoxic Lung Injury by Activating Macrophage Polarization in Neonatal Rat Model

Figure 2

Secretion protein array of UCB-MSCs with altered expression under inflammation conditions. (a) Biotin label-based antibody array analysis using conditioned medium collected from UCB-MSCs alone versus UCB-MSCs cocultured with LPS-treated NR8383 cells. Proteins secreted under each condition evaluated with a proteome profiler. (b) For the 192 proteins upregulated in UCB-MSCs treated with LPS-induced NR8383 cells, functional categories were classified by biological process annotation. (c) Quantification of the optical intensity of anti-inflammation factor. Intensity analysis showed upregulated protein secretion in the UCB-MSC coculture system compared to the UCB-MSCs cultured alone. Protein levels were evaluated as the fold increase, with data normalized to intensity of UCB-MSCs alone, which was defined as 1. PTX3 showed the most significant increase in the UCB-MSC coculture system (black box). (d) To confirm the upregulation of PTX3, PTX3 secretion level was measured in 4 different samples by ELISA for UCB-MSCs alone and for cocultured UCB-MSCs. Error bars represent the , per group; . MФ: macrophage; L: LPS.

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