Soluble PTX3 of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Attenuates Hyperoxic Lung Injury by Activating Macrophage Polarization in Neonatal Rat Model

Figure 3

PTX3 knockdown in UCB-MSCs accelerates macrophage polarization. NR8383 cells were stimulated with LPS and cocultured with UCB-MSCs for 3 days. UCB-MSCs were pretreated with control siRNA or PTX3 siRNA before coculture. (a) Expression of CD11b (red) and CD163 (green) was assessed by quantifying the percentage of positively stained cells. Nuclei were stained with Hoechst 33342. . (b) Cell supernatants were analyzed for inflammatory cytokines (rat IL-6, rat IL-8) or anti-inflammatory cytokine (rat IL-10) by ELISA. (a, b) Error bars represent the , per group; , vs. MФ. ⁺, ⁺⁺ vs. MФ+L. ^ΔΔ vs. MФ+L+naïve MSC. ^◆◆ vs. MФ+L+Con siRNA MSC. MФ: macrophage; L: LPS.

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