Soluble PTX3 of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Attenuates Hyperoxic Lung Injury by Activating Macrophage Polarization in Neonatal Rat Model

Figure 4

Dectin-1 of macrophages was activated by PTX3 of UCB-MSCs under inflammation condition. (a) NR8383 cells were stimulated with LPS and cocultured with UCB-MSCs for 3 days. UCB-MSCs were pretreated with control siRNA or PTX3 siRNA before coculture. The expression of Dectin-1 (green) in NR8383 cells was assessed by quantifying the percentage of positively stained cells. Nuclei were stained with Hoechst 33342. The merged image is an overlay of the Hoechst 33342 and Dectin-1 images. . Error bars represent the , per group; vs. MФ. ⁺, ⁺⁺ vs. MФ+L. ^ΔΔ vs. MФ+L+naïve MSC. ^◆◆ vs. MФ+L+Con siRNA MSC. (b) NR8383 cells were stimulated with LPS and cocultured with UCB-MSCs for 3 days. NR8383 cells were pretreated with control siRNA or Dectin-1 siRNA before coculture. Cell supernatants were analyzed for inflammatory cytokines (rat IL-6, rat IL-8) or anti-inflammatory cytokine (rat IL-10) by ELISA. Error bars represent the , per group; vs. MФ. ⁺, ⁺⁺ vs. MФ+L. ^Δ, ^ΔΔ vs. MФ+L+MSC. ^◆, ^◆◆ vs. Con siRNA MФ+L+MSC. MФ: macrophage; L: LPS.

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