Soluble PTX3 of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Attenuates Hyperoxic Lung Injury by Activating Macrophage Polarization in Neonatal Rat Model

Figure 6

Comparison of therapeutic outcome by PTX3 secretion level using hyperoxic lung injury rat model. (a) NR8383 cells were stimulated with LPS and cocultured with 3 lots of UCB-MSCs for 3 days. PTX3 secretion significantly differed under inflammation conditions following treatments. Error bars represent the , per group; vs. MФ. ⁺⁺ vs. MФ+L. ^ΔΔ vs. MФ+L+MSC5. ^◆◆ vs. MФ+L+MSC6. (b) Daily survival rate during14 days after birth. (c) Histologic and morphometric analysis of rat lung tissue. Representative microscopy photomicrographs of lung tissue with H&E staining. . Degree of alveolarization analyzed by MLI. Error bars represent the , per group; vs. normal. ⁺⁺ vs. BPD. ^ΔΔ vs. BPD+MSC5. (d, e) Representative immunofluorescence staining using macrophage markers (CD11b, CD163) in lung tissue. Expression of CD11b (d, red) and CD163 (e, green) was counted as positively stained cells. (f) Expression of Dectin-1 (green) in lung tissue was counted as positively stained cells. (d–f) Nuclei were stained with Hoechst 33342. . (g) Secretion levels of rat IL-6, rat IL-8, and rat IL-10 in the lung BALF. (d–g) Error bars represent the , per group; , vs. normal, ⁺⁺ vs. BPD, ^ΔΔ vs. BPD+MSC5, ^◆, ^◆◆ vs. BPD+MSC6. MФ: macrophage; L: LPS; BPD: bronchopulmonary dysplasia.

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