Combined Transplantation of Adipose Tissue-Derived Stem Cells and Endothelial Progenitor Cells Improve Diabetic Erectile Dysfunction in a Rat Model

Figure 4

Combined transplantation of ADSCs and EPCs improved the endothelial function of DED rats. (a) Immunofluorescence staining of CD31 (green) and EdU (red) in the cavernous tissue in each group. The white arrow shows the transplanted EPCs. Scale bar: 100 μm. (b) Immunofluorescence staining of eNOS (red) in the cavernous tissue in each group. Scale bar: 50 μm (c, d) The representative images and quantity analysis of western blotting, including CD31 and eNOS. Data is expressed as relative expression compared to β-actin and to the normal group. (e) NO level in the serum of each group determined by ELISA assay. (f) cGMP concentration in the cavernous tissue in each group was determined by ELISA. , . EdU: 5-ethynyl-2-deoxyuridine; eNOS: endothelial nitric oxide synthase; NO: nitric oxide; cGMP: cyclic guanosine monophosphate.

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