Vitamin C Treatment Rescues Prelamin A-Induced Premature Senescence of Subchondral Bone Mesenchymal Stem Cells

Figure 1

Prelamin A accumulation triggered premature senescent phenotype and reduced multidifferentiation potential. (a) Prelamin A accumulated after in vitro subculturing. (b) SA-β-Gal activity increased after passages. Scale bar: 100 μm. (c) Representative confocal images showed nuclear membrane abnormalities, nuclear enlargement, and morphology defects. Scale bar: 50 μm. (d) Prelamin A triggered the increase activity of SA-β-Gal. Scale bar: 100 μm. (e) Immunophenotypic features of stem cell markers in MSC/GFP and MSC/PLA. (f) Representative confocal images (left) and flow cytometry (right) demonstrated the expression of Ki67 in MSC/GFP and MSC/PLA. Scale bar: 100 μm. (g) Comparison of mRNA expression levels of cytokines between MSC/GFP and MSC/PLA (, ). (h) ALP staining (top, left) and alizarin red (bottom, left) demonstrated reduced osteogenic potential in MSC/PLA. Scale bar: 500 μm. Comparison of mRNA expression levels (right) of osteogenic related genes (Runx-2 and OPN, OCN). Oil red staining (I, left) and the mRNA expression levels of PPAR-γ and CEBP/α (I, right) demonstrated adipogenic characteristics in the two groups. independent experiments ().

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