Vitamin C Treatment Rescues Prelamin A-Induced Premature Senescence of Subchondral Bone Mesenchymal Stem Cells

Figure 2

Transplantation of MSC/PLA attenuated the rescue effect of SCB-MSCs in hind-limb ischemia. (a) Experimental paradigm for transplantation study. (b) Representative pictures of limb survival of PBS, MSC/GFP, and MSC/PLA groups. (c) Representative Laser-Doppler flow images (left) post-isch, and at days 0, 7, and 14 of PBS, MSC/GFP, and MSC/PLA, respectively. Quantitative evaluation of blood flow (right) expressed as a ratio of ischemic to normal limb demonstrated a significant increase of limb blood perfusion in the MSC/GFP group, compared with the PBS and MSC/PLA groups. (d) Histological analysis on cross sections in the PBS, MSC/GFP, or MSC/PLA groups in ischemic legs. Hematoxylin and eosin staining (top, left) and quantification (top, right) for infiltration of numerous granulocytes and neutrophils. Scale bar: 50 μm. Masson trichrome staining (middle, left) and quantification (middle, right) visualized fibrosis in hind-limb ischemia. Visualization of neovascularization by immunofluorescence images (bottom, left) and quantification (bottom, right) with CD31 labeling. Scar bar:100 μm (MSC/GFP vs. PBS: , , ; MSC/PLA vs. PBS: ^&, ^&&, MSC/GFP vs. MSC/PLA: ^$).

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