Mesenchymal Stem Cells from Human Exfoliated Deciduous Teeth and the Orbicularis Oris Muscle: How Do They Behave When Exposed to a Proinflammatory Stimulus?

Figure 2

Effects of proinflammatory stimulation with IFN-γ on the osteogenic differentiation potential of SHED and OOMDSCs. (a, b) The addition of IFN-γ at a concentration of 10 ng/mL, 25 ng/mL, 50 ng/mL, 100 ng/mL, or 500 ng/mL maintained the osteogenic differentiation potential of both SHED (a) and OOMDSCs (b) at levels similar to the level observed with control treatment. A statistically significant difference was observed only between SHED maintained in osteogenic differentiation medium supplemented with IFN-γ at a concentration of 500 ng/mL ( sd) and SHED cultured in osteogenic differentiation medium supplemented with IFN-γ at a concentration of 10 ng/mL ( sd). , determined by one-way ANOVA followed by the Bonferroni post hoc test. Five experimental replicates were performed for each group for both SHED and OOMDSCs.

(a)

(b)