Mesenchymal Stem Cells from Human Exfoliated Deciduous Teeth and the Orbicularis Oris Muscle: How Do They Behave When Exposed to a Proinflammatory Stimulus?

Figure 3

Effects of IFN-γ on the proliferative capacity and cell viability of SHED and OOMDSCs. (a) An inhibition of cell proliferation in SHED was observed after three days of culture in culture medium supplemented with IFN-γ at different concentrations. After the fifth day of culture, it was observed that the addition of IFN-γ at higher concentrations (100 ng/mL and 500 ng/mL) to the culture medium resulted in a greater inhibition of the proliferation of SHED than the addition of IFN-γ at lower concentrations (10 ng/mL, 25 ng/mL, and 50 ng/mL); however, this difference was not statistically significant. A similar effect was observed after seven days of cell culture; however, a statistically significant reduction in the cell proliferation of SHED treated with IFN-γ at the 100 ng/mL concentration compared with that of untreated SHED was observed. (c) An inhibition of proliferation in OOMDSCs was observed after five days of culture in culture medium supplemented with IFN-γ at different concentrations. Beginning on the fifth day of culture, it was observed that compared with no IFN-γ addition, the addition of IFN-γ to the culture medium resulted in a statistically significant inhibition of OOMDSC proliferation. After seven days of culture, the addition of IFN-γ at higher concentrations (50 ng/mL, 100 ng/mL, and 500 ng/mL) to the culture medium resulted in a greater inhibition of OOMDSC proliferation; however, compared with the OOMDSCs maintained in culture medium without IFN-γ, only the OOMDSCs treated with IFN-γ at 500 ng/mL had their proliferation significantly inhibited. (b) A statistically significant decrease in the cell viability of SHED was observed only on the third day of culture in culture medium supplemented with IFN-γ at distinct concentrations. Furthermore, a greater decrease in the viability of SHED was detected in the groups treated with IFN-γ at a 25 ng/mL, 50 ng/mL, or 100 ng/mL concentration than in the groups treated with lower concentrations of IFN-γ. No significant differences were observed among the populations of SHED after five or seven days of culture in culture medium supplemented with IFN-γ at distinct concentrations. (d) For OOMDSC populations, a statistically significant decrease in cell viability on the third day of culture was observed only when the cells were treated with IFN-γ at 50 ng/mL, 100 ng/mL, or 500 ng/mL. On the fifth day of culture, however, a statistically significant decrease in cell viability was observed only when OOMDSCs were treated with IFN-γ at 100 ng/mL or 500 ng/mL. Additionally, a statistically significant decrease in cell viability was observed on the seventh day of culture only when OOMDSC populations were treated with IFN-γ at a concentration of 500 ng/mL. , determined by two-way ANOVA followed by the Bonferroni post hoc test. Five experimental replicates were performed for each group for both SHED and OOMDSCs.

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