Mesenchymal Stem Cells from Human Exfoliated Deciduous Teeth and the Orbicularis Oris Muscle: How Do They Behave When Exposed to a Proinflammatory Stimulus?

Figure 4

Effects of IFN-γ on the expression of immunogenic, costimulatory, and immunomodulatory molecules on the cell surface of SHED and OOMDSCs. (a, b) Neither SHED (a) nor OOMDSCs (b) expressed the costimulatory molecules CD40, CD80, and CD86 or the human leukocyte antigen class II HLA-DR on their cell surface, either in the absence of proinflammatory stimulation with IFN-γ or when stimulated with 25 ng/mL IFN-γ. (c) Higher expression of the human leukocyte antigen class I molecules HLA-A, HLA-B, and HLA-C was observed on the cell surface of both SHED and OOMDSCs treated with 25 ng/mL IFN-γ than on that of MSCs that were not treated with IFN-γ. However, this increase in the MFI value was statistically significant only when the OOMDSCs were treated with 25 ng/mL IFN-γ. (d) After treatment with 25 ng/mL IFN-γ, the expression of the immunomodulatory molecule HLA-G by both OOMDSCs and SHED was higher than that in the corresponding control-treated cells. This increase in the MFI value was statistically significant for both the SHED and the OOMDSCs treated with 25 ng/mL IFN-γ. (e, f) The effects of IFN-γ on the cell surface and intracellular expression of HLA-G were assessed. (e) Cell surface HLA-G expression remained unchanged in SHED treated with 25 ng/mL IFN-γ but increased in OOMDSCs treated with 25 ng/mL IFN-γ; however, this increase was not statistically significant. (f) Intracellular HLA-G expression increased significantly in both SHED and OOMDSCs treated with 25 ng/mL IFN-γ. Histograms demonstrate the binding of conjugated antibodies (in red) and isotype controls (in blue) to antigens. , determined by an unpaired Student -test. Five experimental replicates were performed for each group for both SHED and OOMDSCs.

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