Multimodal Therapeutic Effects of Neural Precursor Cells Derived from Human-Induced Pluripotent Stem Cells through Episomal Plasmid-Based Reprogramming in a Rodent Model of Ischemic Stroke

Figure 1

Neural induction of human ep-iPSCs into neural precursor cells. (a) Immunofluorescence staining of undifferentiated markers (OCT4, SOX2, NANOG, SSEA4, TRA-1-60, and TRA-1-81) in induced pluripotent stem cells (ep-iPSCs). (b) Fluorescent-activated cell sorting (FACS) analysis of undifferentiated markers (SSEA4 and TRA-1-60) in ep-iPSCs. (c) Polymerase chain reaction (PCR) of undifferentiated markers (OCT4, NANOG, SOX2, and LIN28) in ep-iPSCs. (d) Double immunofluorescence staining for neural precursor cell (NPC) markers (Nestin and SOX2) in ep-iPSC-NPCs. DAPI (blue) stain nuclei. (e) PCR analysis of NPC markers (Nestin and SOX2) in ep-iPSC-NPCs. Scale bars: 50 μm. ep-iPSC-NPCs: neural precursor cells differentiated from induced pluripotent stem cells.

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