Multimodal Therapeutic Effects of Neural Precursor Cells Derived from Human-Induced Pluripotent Stem Cells through Episomal Plasmid-Based Reprogramming in a Rodent Model of Ischemic Stroke

Figure 3

Differentiation of grafted ep-iPSC-NPCs in the brains of MCAo rats. (a) Double immunofluorescence staining for neuronal differentiation markers (MAP2, NeuN, Darpp32, GABA, and GAD65/67) in ep-iPSC-NPC-transplanted brain. Human-specific nuclear (hNu) and mitochondrial (hMito) markers were used to detect grafted cells. (b) Double immunofluorescence staining for glial differentiation markers (GFAP and O4) in the ep-iPSC-NPC-transplanted brain. The human mitochondrial marker (MTC02) was used to detect grafted cells. The human-specific marker (hNu) was used to detect grafted cells. All samples were counterstained with DAPI. Scale bars: 50 μm. ep-iPSC-NPCs: neural precursor cells differentiated from induced pluripotent stem cells; MCAo: middle cerebral artery occlusion.

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