Multimodal Therapeutic Effects of Neural Precursor Cells Derived from Human-Induced Pluripotent Stem Cells through Episomal Plasmid-Based Reprogramming in a Rodent Model of Ischemic Stroke

Figure 5

Subventricular zone neurogenesis analyses in MCAo rats with ep-iPSC-NPC transplantation. (a) Double immunofluorescence staining of PCNA/PSA-NCAM in the SVZ of rats in the ep-iPSC-NPC, fibroblast, and vehicle groups ( in each group). (b) Double immunofluorescence staining of BrdU/DCX in the SVZ of the ep-iPSC-NPC, fibroblast, and vehicle groups ( in each group). LV indicates lateral ventricle. All samples were counterstained with DAPI (blue). Scale bars: 50 μm. Data are shown as (SEM), by one-way ANOVA. BrdU: 5-bromo-2-deoxyuridine; DCX: doublecortin; ep-iPSC-NPCs: neural precursor cells differentiated from induced pluripotent stem cells; MCAo: middle cerebral artery occlusion; PCNA: proliferating nuclear antigen; PSA-NCAM: polysialic acid-neural cell adhesion molecule; SVZ: subventricular zone.

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