CD14 and ALPK1 Affect Expression of Tight Junction Components and Proinflammatory Mediators upon Bacterial Stimulation in a Colonic 3D Organoid Model

Figure 4

Expression of proinflammatory mediators after LPS stimulation. Changes in gene expression of cytokines and their supernatant concentration from organoids stimulated with LPS for 6 hours were analysed. Knockout organoids were compared to wild type (WT). Values are normalized to unstimulated controls within each genotype for each experiment and represent the means with 95% CI. Unstimulated: WT , Il10^-/-, Cd14^-/-, and Alpk1^-/-. Stimulated: WT , Il10^-/-, Cd14^-/-, and Alpk1^-/-. (a) Tnfα/TNFα, (b) Cxcl1/CXCL1, (c) Cxcl2/CXCL2, and (d) Cxcl5/CXCL5. Stimulated versus unstimulated values were compared using unpaired -tests. In the case of unequal variances, Brown-Forsythe and Welch ANOVA test was performed. In all other cases, a one-way analysis of variance followed by Dunnett’s multiple comparisons was performed. Each knockout genotype was compared to WT. , , , and .

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