Human Adipose Derived Cells in Two- and Three-Dimensional Cultures: Functional Validation of an In Vitro Fat Construct
A comparison of the in vitro behavior of human SVF cells when cultured in 2-dimensional conditions and in 3 dimensions when seeded on either silk scaffolds or cultured in ObaGel for 2 weeks. (a) Confocal and cryoscanning electron microscopy (cryo-SEM) images of SVF cell seeding, proliferation, and adipogenic differentiation behavior in traditional 2D culture, silk scaffolds, and ObaGel. (b) SVF immunophenotype following 1 week of culture reveals that ObaGel is superior in maintaining a heterogenic immunophenotype similar to freshly isolated cells and supports the expansion of CD44-positive subpopulations (b), and adipokine functionality (c) reports the measure of leptin secretion from adipogenic differentiated cells seeded on silk or with ObaGel are early (day 3) and late (day 28) time points of differentiation. Immunophenotype values reported as (SD). .
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