Research Article
Acute Myocardial Infarction Reduces Respiration in Rat Cardiac Fibers, despite Adipose Tissue Mesenchymal Stromal Cell Transplant
Figure 2
High-resolution respirometry profile of mitochondria in permeabilized cardiac fibers. (a) Representative profile of the O2 consumption by using permeabilized cardiac fibers from Sham-operated rats. (b) O2 consumption in the border zone (BZ) from rats subjected to AMI; this record was obtained 24 h after AMI, which corresponds to the necrotic phase. (c) O2 consumption in the infarcted zone (IZ) from rats subjected to AMI; this record was obtained 24 h after AMI. The O2 consumption normalized by tissue mass (black trace) was measured using high-resolution respirometry. Different mitochondrial function parameters were calculated following the multiple substrate-uncoupler-inhibitor titration (SUIT) protocol (arrows), as described in High-Resolution Respirometry. Gray trace represents the O2 concentration in the media.
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