Acute Myocardial Infarction Reduces Respiration in Rat Cardiac Fibers, despite Adipose Tissue Mesenchymal Stromal Cell Transplant

Figure 3

The scrutiny of mitochondrial respiration in the ischemic and border zones, at the necrotic and fibrotic phases, after myocardial infarction (PBS group). The mitochondrial functional parameters described in each panel were obtained or calculated with the use of different substrates in nonphosphorylating and phosphorylating conditions, with specific inhibitors, in the uncoupled state (SUIT protocol) and after addition of cytochrome c, as described in High-Resolution Respirometry. Assays were carried out using permeabilized cardiac fibers. Sham: animals underwent open chest surgery according to the methods. The infarcted zone (IZ) and the border zone (BZ) were analyzed separately. Samples were analyzed 24 h (necrotic phase) or 8 days (fibrotic phase) after AMI induction. Groups are indicated in the abscissa. Respiratory rates are expressed as the O₂ flux normalized by the muscle wet weight (pmol·s^-1·mg^-1) (a–i). Cytochrome c (j) is represented as the percentage. Data represent values. The number of animals in each group was as follows: Sham group, ; PBS-24 h after infarction, ; and PBS-8 d after infarction, . ^# with respect to Sham; when comparisons are made within the 4 BZ and IZ subgroups (dashed lines), the levels of significance are indicated by the number of asterisks: ; ; (one-way ANOVA with Bonferroni’s posttest).

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)