Stem Cells International

Research Article

Acute Myocardial Infarction Reduces Respiration in Rat Cardiac Fibers, despite Adipose Tissue Mesenchymal Stromal Cell Transplant

Table 1

Effects of mesenchymal stromal cells from adipose tissue (AD-MSCs) on the mitochondrial function of permeabilized cardiac fibers (BZ) from rats after acute myocardial infarction (AMI). Rats were treated with AD-MSCs or PBS after AMI, and different parameters of the mitochondrial function of permeabilized cardiac fibers were analyzed by high-resolution respirometry, according to the multiple substrate-uncoupler-inhibitor titration protocol.
Mitochondrial function parameter (in pmol O2 s-1 mg-1)Necrotic phaseFibrotic phase
PBS
()		AD-MSC
()		PBS
()		AD-MSC
()
CI
CIp
Leak state ()
()
CImax
CIImax
ROX
Cyto C (%)
L/E
RCR
OXPHOS coupling efficiency
CI: nonphosphorylative state; CIp: phosphorylative state associated with complex I; : maximal phosphorylative state; (): flux coupled with ATP synthesis; : maximal respiratory capacity; CImax: complex I contribution to the maximal ETS capacity; CIImax: complex II contribution to the maximal ETS capacity; ROX: residual oxygen consumption; Cyto C: an index of mitochondrial outer membrane integrity; : leak control ratio; (: expressing how much from ETS capacity is used to produce ATP; RCR: respiratory control ratio. Values are expressed as the . Any difference was visible between the vehicle-treated (PBS) group and the AD-MSC-treated group (by unpaired -test).