Highly Proliferative Immortalized Human Dental Pulp Cells Retain the Odontogenic Phenotype when Combined with a Beta-Tricalcium Phosphate Scaffold and BMP2

<div>Immortalization of human dental pulp cells using the piggyBac transposon system. (a) Schematic diagram of pMPH86. The SV40 T-Ag gene is located between two flippase recognition target (FRT) sites. (b) Schematic of the experimental approach. (c) IHDPC establishment. The adenoviral vectors AdpBase and pMPH86 transfected the primary human dental pulp cells, and cells were selected in medium with hygromycin for 3 days. Surviving cells were observed at day 3, day 7, and day 13 postselection. The iHDPCs passaged consecutively for 25 passages (p25) (d and e). The mRNA expression of SV40T-Ag was upregulated after immortalization.</div>

Stem Cells International

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Figure 1

Figure 1: Highly Proliferative Immortalized Human Dental Pulp Cells Retain the Odontogenic Phenotype when Combined with a Beta-Tricalcium Phosphate Scaffold and BMP2 

Figure 1 | Highly Proliferative Immortalized Human Dental Pulp Cells Retain the Odontogenic Phenotype when Combined with a Beta-Tricalcium Phosphate Scaffold and BMP2 