Highly Proliferative Immortalized Human Dental Pulp Cells Retain the Odontogenic Phenotype when Combined with a Beta-Tricalcium Phosphate Scaffold and BMP2

Figure 6

IHDPCs can differentiate into odontoblasts when combined with a beta-tricalcium phosphate scaffold and growth factor in vivo. (a) BMP2 promotes the differentiation and mineralization of iHDPCs and dHDPCs in vitro. ALP staining of iHDPCs and dHDPCs after culture in odontogenic medium for 5 days. (b) Composites of scaffolds and cells were carefully implanted into the dorsal subcutaneous region of nude mice for 4 weeks. (c) H&E staining of the composites of β-TCP scaffolds and iHDPCs/dHDPCs treated with BMP2 after subcutaneous implantation for 4 weeks. (d) Masson’s trichrome staining of the composites. (e) The levels of DSPP in the β-TCP scaffold and iHDPC/dHDPC composites were analyzed by immunocytochemical staining.

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