Curcumin-Activated Mesenchymal Stem Cells Derived from Human Umbilical Cord and Their Effects on MPTP-Mouse Model of Parkinson’s Disease: A New Biological Therapy for Parkinson’s Disease

Figure 3

Experimental schema, behavioral changes, and MSC tracking. (a) Schema detailing method for establishment of PD mouse model and infusion MSC; the number in the box is the rest of mice. (b) Open field test. The average number of rearing in the model and F12 groups was decreased compared with that in the normal group (). The numbers of rearing in the MSC and MSC-CUR groups were significantly improved compared with the model group (). Significant difference was also found between the MSC group and the MSC-CUR group (). Changes of ambulation were consistent with that of rearing. (c) Rotarod test. The tendency of latency to fall was similar to that of rearing. Changes of drop frequency within 2 min were opposite to the changes of latency to fall. The drop frequency was increased due to limb stiffness and imbalance of movement in the model and F12 groups compared with the normal group (). Significant improvement was observed after stem cell treatment (). Moreover, the improvement was more important in the MSC-CUR group compared with the MSC group () (one-way analysis of variance, Bonferroni’s multiple comparisons test, , , ). (d) Under LSCM, MSCs labeled with DiI (red fluorescence, shown as arrows) were presented in the striatum region of MSC and MSC-CUR group mouse brain; the change of TH expression can be clearly seen (green fluorescence).

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