Curcumin-Activated Mesenchymal Stem Cells Derived from Human Umbilical Cord and Their Effects on MPTP-Mouse Model of Parkinson’s Disease: A New Biological Therapy for Parkinson’s Disease

Figure 8

Detection of TH and MAP2 in SH-SY5Y cells treated with CM-CUR and CM-MSC by immunofluorescence and western blot. (a) TH and MAP2 expression was upregulated in SH-SY5Y cells treated with CM-MSC and CM-CUR. Obvious morphological changes were observed in SH-SY5Y cells. The cells had many long processes as long as more than 2-fold that of the cell. These processes were similar in morphology to axons (arrows, ). These morphological changes were remarkable in the CM-CUR group. (b) Image and statistical analyses of (a). TH and MAP2 expression in the normal control was stronger than that in the model group (). TH and MAP2 expression was increased after treatment with CM-MSC and CM-CUR (). Significant difference in upregulation of MAP2 expression was observed between the CM-MSC and CM-CUR groups (). (c, d) The expression of TH and MAP2 was detected by western blot. The pattern was similar to that detected by immunofluorescence (one-way analysis of variance, Bonferroni’s multiple comparisons test, , , ). All experiments were performed thrice.

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