Hypoxic Wharton’s Jelly Stem Cell Conditioned Medium Induces Immunogenic Cell Death in Lymphoma Cells

Figure 3

Mitochondria stress assay of lymphoma cells after treatment with normoxic and hypoxic hWJSC-CM. (a) Note significant increases in mitochondria superoxide level of lymphoma cells after treatment with hWJSC-CM as compared to control. Treatment with hWJSC-CM collected under 5% oxygen conditions showed the highest increase in lymphoma mitochondria superoxide levels. (b) Note significant decreases in mitochondria membrane potential of lymphoma cells after treatment with hWJSC-CM as compared to the control. Treatment with hWJSC-CM collected under 5% oxygen conditions showed the highest decrease in mitochondria membrane potential. (c) Note significant increases in the lipid peroxidation level of lymphoma cells after treatment with hWJSC-CM as compared to the control. Treatment with hWJSC-CM collected under 5% oxygen conditions showed the highest increase in lipid peroxidation. All values are expressed as the of 3 biological samples with 3 replicates for each sample. .

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