Research Article
Actin Alpha 2 (ACTA2) Downregulation Inhibits Neural Stem Cell Migration through Rho GTPase Activation
Figure 3
ACTA2 downregulation inhibited NSC migration. (a) RT-PCR showing ACTA2 mRNA expression with ACTA2 siRNA transfection. (b) Quantification of ACTA2 mRNA expression from (a) ( for each group). , one-way ANOVA followed by Tukey’s post hoc test. (c) Bands showing ACTA2 protein expression with ACTA2 siRNA transfection. (d) Quantification of ACTA2 protein expression from (c) ( for each group). , , one-way ANOVA followed by Tukey’s post hoc test. (e) Representative images of NSC migration from neurospheres plated on PO-precoated 24-well plates under different conditions after 12 hours. Scale bar: 100 μm. (f) Bar graph summarized the number of migration cells from neurospheres in each group ( for each group). , one-way ANOVA followed by Tukey’s post hoc test. (g) Bar graph summarized the average outgrowth distance migrating from neurospheres in each group ( for each group). , one-way ANOVA followed by Tukey’s post hoc test.
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