Stromal Cell-Derived Factor-1 Enhances the Therapeutic Effects of Human Endometrial Regenerative Cells in a Mouse Sepsis Model

Figure 1

Pretreatment with SDF-1 on ERCs promoted SDF-1 and CXCR4 expression and further improved sepsis symptoms. (a) The expression of human CXCR4 on the surface of ERCs pretreated with SDF-1 at 0 ng/ml and 50 ng/ml for 72 hours. Statistical analysis was performed by Mann-Whitney , , and . (b) The mRNA expression levels of murine SDF-1 and human CXCR4 in liver (i) and lung (ii) tissues. Statistical analysis was performed by one-way analysis of variance (ANOVA), and the posttest was the least significant difference (LSD) test, . , , and . (c) The survival rate (i), mean rectal temperature (ii), and mean murine sepsis score (iii). There was no death found in normal control group, unmodified ERC group, and SDF-1-pretreated ERC group until the end point of the observation (i). Statistical analysis was performed by one-way analysis of variance (ANOVA), and the posttest was the least significant difference (LSD) test, . , , and (SDF-1-pretreated ERC group vs. unmodified ERC group). ^#, ^##, and ^### (AMD3100-pretreated ERC group vs. unmodified ERC group). Bar graphs represent . Abbreviation: ERCs: endometrial regenerative cells.

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