Antibiotics Disturb Dentin Formation and Differentiation of Dental Pulp Stem Cells: The Role of Microbiota in Cellular Turnover of Mouse Incisor

Figure 2

Histological analysis of the apical area and dentin formation of incisors. (a) H&E staining showed the histological structure of the apical area. (b) The distance was measured as the sketch map has shown; point stands for epithelial invagination, and point stands for mineralized dentin reaching a width of 10 μm. (c) The distance between points and was calculated, and it showed a longer distance in AbT. (d, e) Immunohistochemical staining of DSPP showed delayed mineralization of dentin in the AbT group. (f, g) Immunohistochemical staining of Ki-67 showed that at 12 hours after injury, Ki-67-positive cells began to accelerate (the second panel), and acceleration of Ki-67-positive cells reached the peak at 24 hours after injury (the third panel), and at a 72-hour time point, the amount of Ki-67-positive cells descended to almost the same level as the 12-hour time point (the right panel) ( μm). All experimental data were verified in at least three independent experiments. Error bars represent the SEM from the mean values. ; ; ; . AbT: antibiotic treated; ConvD: conventionalized; SPF: specific pathogen-free.

(a)

(b)

(c)

(d)

(e)

(f)

(g)