Antibiotics Disturb Dentin Formation and Differentiation of Dental Pulp Stem Cells: The Role of Microbiota in Cellular Turnover of Mouse Incisor

Figure 3

DPSCs from AbT mice exhibited impaired colony-forming ability and mineralized potential. (a) Colony-forming unit experiments showed that DPSCs derived from AbT mice had lower colony-forming rate compared to those from SPF and ConvD mice. (b) Alizarin red staining showed that AbT-derived DPSCs formed less mineralized nodule in vitro compared to SPF and ConvD groups. (c) Real-time PCR results showed that SPF-DPSCs exhibited higher expression of mineralization relative mRNA. (d) Western blot analysis showed that AbT-DPSCs expressed lower DSPP after osteo-/odontogenic induction in vitro. All experimental data were verified in at least three independent experiments. Error bars represent the SEM from the mean values. ; ; ; . AbT: antibiotic treated; ConvD: conventionalized; SPF: specific pathogen-free; DSPP: dentin sialophosphoprotein.

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