5-Azacytidine-Induced Cardiomyocyte Differentiation of Very Small Embryonic-Like Stem Cells

Figure 1

Rat bone marrow- (BM-) derived very small stem cells (VSELs) were successfully isolated by flow cytometry. (a) The tibia and femur of rats were separated, erythrocytes were lysed, and total BM mononuclear cells were extracted. Fluorophore-conjugated antibodies specific to CD45, CD106, and hematopoietic markers (Lin: TCRαβ, CD3, CD11b, and CD45RA) were used. Flow cytometric sorting was carried out after incubation. (b) Blank control; (c) Lin control; (d) CD45 control; (e) CD106 control; (f) VSEL sorting.

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